MersV1, Main, Exploration, bibRecord, 004F99

Polyribosomes in protoplasts isolated from tobacco leaves

Identifieur interne : 004F99 ( Main/Exploration ); précédent : 004F98; suivant : 005000

Polyribosomes in protoplasts isolated from tobacco leaves

Auteurs : P. Ruzicska [Hongrie] ; R. Mettrie [Hongrie] ; Y. L. Dorokhov [Hongrie] ; G. Premecz [Hongrie] ; T. Oláh [Hongrie] ; G. L. Farkas [Hongrie]

Source :

Planta [ 0032-0935 ] ; 1979-01-01.

RBID : ISTEX:623043FE171838BB5C87856E84711244AFF848E9

English descriptors

KwdEn :
- Nicotiana, Polysomes, Protein synthesis, Protoplasts, Ribonuclease, Ribosomes, Stress.
Teeft :
- Callus, Extraction medium, Farkas, Larkins, Leaf tissue, Leaf tissues, Osmotic stress, Physiol, Plant physiol, Plant protoplasts, Polyribosomes, Polysome, Polysome classes, Polysome preparations, Polysome profiles, Polysomes, Premecz, Protein synthesis, Protoplast, Ribosomal subunits, Rnase, Sucrose density gradient profiles, Tobacco leaf protoplasts, Tobacco protoplasts.

Abstract

Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.

Url:

https://api.istex.fr/ark:/67375/1BB-FDX4JMVK-K/fulltext.pdf

DOI: 10.1007/BF00388718

Affiliations:

Hongrie

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000101
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to stream Main, to step Curation: 004F99

Le document en format XML

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<term>Polysome classes</term>
<term>Polysome preparations</term>
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<term>Polysomes</term>
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<term>Protein synthesis</term>
<term>Protoplast</term>
<term>Ribosomal subunits</term>
<term>Rnase</term>
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<front><div type="abstract" xml:lang="en">Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</div>
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Polyribosomes in protoplasts isolated from tobacco leaves

Polyribosomes in protoplasts isolated from tobacco leaves

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri